Dmso X-gal

5-BROMO-4-CHLORO-3-INDOLYL b-D-GALACTOPYRANOSIDE Product Number B4252, B9146, B6024 Storage Temperature −20°C Cas #: 7240-90-6 Synonyms: X−Gal Product Description 5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside, commonly known as X−Gal, is a histochemical substrate for β−galactosidase.1 X−Gal is cleaved by

20 mg/ml solution. It must be dissolved in DMSO (dimethyl sulfoxide) or dimethyl formamide, not water! It must be wrapped in foil to protect it from the light, sterilize by filtration, and then stored in the freezer. Using IPTG and X-gal for blue/white selection on Petri plates

American Journal of Respiratory Cell and Molecular Biology – LacZ activity was determined by 5-bromo-4-chloro-3-indolyl B-D-galactopyranoside (X-gal) (Sigma, St. Louis, MO) staining. X-gal–stained sections were processed for immunofluorescence for localization.

X-gal: 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside. Stock Solution Concentration: 20 mg/ml To 100 mg X-gal add 5 ml dimethylformamide (DMF) or DMSO and store at -20C. It must be dissolved in DMSO (dimethyl sulfoxide) or dimethyl formamide, not water! It must be wrapped in foil to protect it from the light, and stored in the freezer.

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A final DMSO concentration of 0.1% was not exceeded in the screening.

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citric acid-sodium phosphate buffer (40–60 mM, pH 6.0), 1 mM of X-gal (Gold biotechnology), 0.1 mM of nitro blue tetrazolum.

Blue White Screening of Bacterial Colonies - X Gal / IPTG PlatesThe X-gal and P-gal assays both yielded approxi- mately the same MF values in the liver DNA of ENU-treated mice. The X-gal values were consis- tently lower than the P-gal values, but the difference was not statistically significant. Both systems showed similar levels of mutant induction after 14 days.

1. Biotechniques. 1993 Feb;14(2):234. Substitution of DMSO for DMF as a solvent for X-gal. Edwards MJ(1), Taylor MF. Author information: (1)Department of Entomology, University of Arizona, Tucson 85721.

Thermo Scientific X-Gal (5-bromo-4-chloro-3-indolyl-beta-D-galacto-pyranoside) is an inert chromogenic substrate for beta-galactosidase which hydrolyzes X-Gal into colorless galactose and 4-chloro-3-brom-indigo, forming an intense blue precipitate.

This protocol describes the preparation of a 5-bromo-4-chloro-3-indolyl β-D-galactopyranoside (X-gal) stock solution at various concentrations. Typically used in conjunction with IPTG for blue/white screening via the lac operon. A typical stock concentration of X-gal is 50mM (20mg/mL).To apply X-gal directly to the top of agar plates for blue/white screening, apply 40μL and wait to dry.

What would be the suitable storage conditions for X-gal dissolved in DMSO? The data sheet provided with my X-gal powder is stunningly cryptic. It mentions that X-gal stock solutions should.

2) X-gal Stock Solution (40X): 40 mg/ml in DMSO (100 mg in 2.5 ml DMSO) (store at -20 deg, protected from light) 3) Final X-gal Solution: Dilute X-gal stock solution 1:40 in Solution A. (first warm Solution A to 37 degrees C to prevent precipitation of X-gal) 4) Formalin/Glutaraldehyde fixative: 12.3 ml distilled water; 10.0 ml 1% glutaraldehyde

Dmso Dosage For Humans Jan 17, 2019. Here are a few interesting facts about DMSO's history and action. In human medicine, these include use as an organ preservative and as treatment for a bladder disease. . Scant information is available regarding dosing. Dimethyl sulfoxide (DMSO) has long been recognized as an important reaction solvent for the synthesis of drug

and 2 mg/ml of X-gal. Some slides were then immunostained for pan-cytokeratin antigen, as described below. Elastin degradation in BAL cells or primary rat type II epithelial cells was examined using.

LN and PBMC were stored in 500 μl medium containing interleukin (IL)-2 and 500 μl of 20% DMSO/RF10 (LN) and 500 μl FCS (PBMC.

Agar plates containing 100 mg/ml ampicillin, 40 mg/ml X-gal and 20.

Dmso Cancer Treatment It is experimental in the sense that no one knows yet how to convert what was discovered in the lab into an actual cancer treatment. It is also not known whether MSM actually helps the DMSO revert cancer cells into normal cells. DMSO, by itself, is said to revert many types of cancer cells into

erol, DMSO, X-gal (20-40 gml 1), spectinomycin (25 g ml 1), chloramphenicol (10 gml 1), and tetracycline (7.5 g ml 1). Mutant colonies with increased expression of the reporter dcuB-lacZ were blue in color compared with white —) 1-,-

X-gal staining for β-galactosidase activity was performed on HCEC treated with rapamycin 2 nM and control (DMSO) at week two and week five. Each sample was rinsed twice with PBS, fixed in 3.7% PFA for.

X-Gal-IPTG Solution X-Gal-IPTG Solution is a ready-to-use, non-toxic solution that facilitates handling and saves time while providing optimal blue/white colony screening. The solution does not contain DMF or DMSO. X-Gal-IPTG Solution is compatible with commonly used lacZ expression systems in bacteria, yeast and mammalian cells. Storage:

When I was inexperienced, one of my very mistakes was to filter sterilise DMSO without diluting it. To my horror, the filter dissolved! So, what I do now is to dilute the DMSO (for common lab use should be OK) in my culture media to achieve the final concentration, and then filter sterilise the mixture.

or DMSO. X-Gal-IPTG Solution is compatible with commonly used lacZ expression systems in bacteria, yeast and mammalian cells. Storage: The formulation is stable when stored at 4°C. Once added to agar, plates are stable for up to 6 months when stored in the dark at 4°C Testing:

So you can select the white colonies for subsequent screening. X-gal: dissolved in DMSO; working concentration: 20ug/ml; should store in an amber tube (light-sensitive) IPTG: working concentration.

For that, I need to plate the culture with X-Gal and IPTG. X-Gal can be added to the agar plates by pouring it directly on the surface or adding it to a top agar layer.

ResearchGate's Q&A.

Description X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactoside) is a histochemical substrate for β-galactosidase. This substrate yields a blue precipitate upon hydrolysis, making it suitable for use in immunoblotting or immunocytochemical assays.

X-Gal SAFETY DATA SHEET Supersedes Revision: 02/03/2015 Section 4. First Aid Measures Description of First Aid Measures: Hold eyelids apart and flush eyes with plenty of water for at least 15 minutes. Have eyes examined and tested by medical personnel. In Case of Eye Contact: Immediately wash skin with soap and plenty of water for at least 15.

Thermo Scientific X-Gal (5-bromo-4-chloro-3-indolyl-beta-D-galacto-pyranoside) is an inert chromogenic substrate for beta-galactosidase which hydrolyzes X-Gal into colorless galactose and 4-chloro-3-brom-indigo, forming an intense blue precipitate.
Apr 25, 1995.

Whole Mount X-gal Histochemistry of Transgenic Animal Tissues.

happens much more slowly to stocks prepared in DMF than in DMSO. 5.