5-BROMO-4-CHLORO-3-INDOLYL b-D-GALACTOPYRANOSIDE Product Number B4252, B9146, B6024 Storage Temperature −20°C Cas #: 7240-90-6 Synonyms: X−Gal Product Description 5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside, commonly known as X−Gal, is a histochemical substrate for β−galactosidase.1 X−Gal is cleaved by
The golden silk spun by Indian golden silkmoth Antheraea assama, is regarded for its shimmering golden.
The cells were spread-plated on LB-Amp plates (Amp and X-gal each, 100 μg/mL), incubated at.
It offers time and material savings for both experienced and novice investigators. It also eliminates handling of hazardous DMSO or DMF. X-gal/IPTG Solution is.
Cur was dissolved with dimethyl sulfoxide (DMSO) and the storage concentration was 20 mM/ml.
galactosidase staining kit according to the manufacture’s introduction. X-Gal was used as a substrate.
We compared the aging BMSCs treated with apocynin (100 μM) with the DMSO-treated negative control and young BMSCs.
activity SA-β-gal was visualised using X-gal.
Description X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactoside) is a histochemical substrate for β-galactosidase. This substrate yields a blue precipitate upon hydrolysis, making it suitable for use in immunoblotting or immunocytochemical assays.
was detected by the accumulation of blue-violet X-gal crystals in FLO-treated L cells (Fig. 5D). The morphology of these FLO-treated cells was changed to the more flattened and irregular shape.
Yeast two-hybrid screening of proteins interacting with plasmin receptor subunit: C-terminal fragment of annexin A2 – Positive clones were initially selected and then assayed for lacZ activity using a filter β-galactosidase assay with X-Gal. Plasmids from positive yeast.
and then 70 μL sterile DMSO (final.
erol, DMSO, X-gal (20-40 gml 1), spectinomycin (25 g ml 1), chloramphenicol (10 gml 1), and tetracycline (7.5 g ml 1). Mutant colonies with increased expression of the reporter dcuB-lacZ were blue in color compared with white —) 1-,-
Anhydrotetracycline, ATC, 0.1 mg/mL, 100 ng/mL, 1,000×, DMSO, link, Can also.
Bromo-chloro-indolyl-galactopyranoside, X-gal, 40 mg/mL, 80 µg/mL, 500×.
USA), was prepared freshly in dimethyl sulphoxide (DMSO) at a final concentration of 0.1%. (v/v). MGMT, O 6-benzylguanine (BG), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT),
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The β-Galactosidase staining kit utilizes X-gal as the substrate. II. Kit Contents.
Prepare X-gal Solution: Dissolve 20 mg X-gal in 1 ml DMSO or DMF (N-N-.
X-Gal Solution – Novagen Convenience for blue/white screening – Find MSDS or.
X-Gal Solution is provided as a convenient 40 mg/ml concentrate in DMSO,
The cells were then treated with varying amounts of DMSO, PS-341, TRAIL, or the combination.
The stain was then gently aspirated and the wells were washed three times with 1 × PBS. X-Gal staining.
or DMSO. X-Gal-IPTG Solution is compatible with commonly used lacZ expression systems in bacteria, yeast and mammalian cells. Storage: The formulation is stable when stored at 4°C. Once added to agar, plates are stable for up to 6 months when stored in the dark at 4°C Testing:
For that, I need to plate the culture with X-Gal and IPTG. X-Gal can be added to the agar plates by pouring it directly on the surface or adding it to a top agar layer.
ResearchGate's Q&A.
We delivered library compounds 2 h after plating as DMSO solution to a final concentration of 5 μg.
We detected lacZ (β-galactosidase) expression in cells using X-gal staining, or alternatively we.
X-Gal is also known 5-Bromo-4-chloro-3-indolyl-β-D-galactopyranoside and it is used to detect β-galactosidases. The lac Z gene encodes β-galactosidases.
Make 8% X-gal in DMSO. (You can use DMSF instead, but it is more toxic.) Divide into 200 l aliquots and freeze at –20 degrees C. X-gal is expensive stuff, but.
This protocol describes the preparation of a 5-bromo-4-chloro-3-indolyl β-D-galactopyranoside (X-gal) stock solution at various concentrations. Typically used in conjunction with IPTG for blue/white screening via the lac operon. A typical stock concentration of X-gal is 50mM (20mg/mL).To apply X-gal directly to the top of agar plates for blue/white screening, apply 40μL and wait to dry.
What would be the suitable storage conditions for X-gal dissolved in DMSO? The data sheet provided with my X-gal powder is stunningly cryptic. It mentions that X-gal stock solutions should.
Prepare at 40 mg/ml in DMSO. X-gal stock should be stored in the dark at.
Plate HeLa-CD4-LTR-ß-gal cells at 0.4 x 105 cells per well (24 well plate) or at 0.8 x.
Although X-gal is well known reagent to detect β-galactosidase in cell or tissue samples, the.
SPiDER-βGal DMSO stock solution (1 μl) was added to the tube.
Array
and 2 mg/ml of X-gal. Some slides were then immunostained for pan-cytokeratin antigen, as described below. Elastin degradation in BAL cells or primary rat type II epithelial cells was examined using.