USA), was prepared freshly in dimethyl sulphoxide (DMSO) at a final concentration of 0.1%. (v/v). MGMT, O 6-benzylguanine (BG), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT),
Stock solutions 50 mg dissolved in a small amouth of DMSO 200mg in 10mL.
use 40uL on each plate X-gal 40 mg/mL 400 mg X-gal in 10mL DMSO (store at.
Description X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactoside) is a histochemical substrate for β-galactosidase. This substrate yields a blue precipitate upon hydrolysis, making it suitable for use in immunoblotting or immunocytochemical assays.
We delivered library compounds 2 h after plating as DMSO solution to a final concentration of 5 μg.
We detected lacZ (β-galactosidase) expression in cells using X-gal staining, or alternatively we.
indolyl-/3-D-galactopyranoside (X-gal) colour-screening procedure.
. MF× 10 6 Mean_+SD phages mutants. X-gal method. DMSO. 1.5 h. 69455 6. 87. 44+37.
Feb 4, 2019.
View and buy high purity X-Gal from Tocris Bioscience. Used in cloning.
5510 – X-Gal | C14H15BrClNO6 | CAS No. 7240.
DMSO, 40.86, 100.
X-gal is an organic compound consisting of galactose linked to a substituted indole. The compound was synthesized by Jerome Horwitz and collaborators in Detroit, MI, in 1964. The formal chemical name is often shortened to less accurate but also less cumbersome phrases such as bromochloroindoxyl.
The golden silk spun by Indian golden silkmoth Antheraea assama, is regarded for its shimmering golden.
The cells were spread-plated on LB-Amp plates (Amp and X-gal each, 100 μg/mL), incubated at.
It offers time and material savings for both experienced and novice investigators. It also eliminates handling of hazardous DMSO or DMF. X-gal/IPTG Solution is.
The β-Galactosidase staining kit utilizes X-gal as the substrate. II. Kit Contents.
Prepare X-gal Solution: Dissolve 20 mg X-gal in 1 ml DMSO or DMF (N-N-.
Cur was dissolved with dimethyl sulfoxide (DMSO) and the storage concentration was 20 mM/ml.
galactosidase staining kit according to the manufacture’s introduction. X-Gal was used as a substrate.
Prepare at 40 mg/ml in DMSO. X-gal stock should be stored in the dark at.
Plate HeLa-CD4-LTR-ß-gal cells at 0.4 x 105 cells per well (24 well plate) or at 0.8 x.
An extracellular matrix microarray for probing cellular differentiation – When using this buffer and cleaning the pins by dipping them in dimethyl sulfoxide (DMSO) between wells and subjecting them.
Cell arrays were fixed for 20 min in 0.5% glutaraldehyde and stained in.
Thermo Scientific X-Gal (5-bromo-4-chloro-3-indolyl-beta-D-galacto-pyranoside) is an inert chromogenic substrate for beta-galactosidase which hydrolyzes X-Gal into colorless galactose and.
Jump to navigationJump to search. List of reagents is not yet complete. (Bruce Conklin and David Sanan, Gladstone/UCSF). Materials: Potassium Ferrocyanide Crystalline (Fisher Scientific #P232-500. Potassium Ferrocyanide Trihydrate (Fisher Scientific #P236-500).
Anhydrotetracycline, ATC, 0.1 mg/mL, 100 ng/mL, 1,000×, DMSO, link, Can also.
Bromo-chloro-indolyl-galactopyranoside, X-gal, 40 mg/mL, 80 µg/mL, 500×.
Frequently Asked Questions on X-GAL. My X-GAL arrived after days, and was left at room temperature. Is the product still good acceptable to use? Most of our product is solid.
It must be dissolved in DMSO (dimethyl sulfoxide) or dimethyl formamide, not water! Spread 40 ul of IPTG and 40 ul of X-gal on top of the plate with a hockey stick spreader.
Stable X-gal/IPTG non-toxic solution for use in blue/white colony screening in expression studies. It does not contain the toxic DMF and DMSO. The solution.
Dmso Zebrafish Toxicity DMSO has been shown to be the most potent inducer of stress proteins. Based on the study, the chemicals tested here may be used as carrier solvents in the zebrafish embryo assay at levels below. . toxicity and proteotoxicity of this drug on the growing fish embryos, eggs of zebrafish were exposed to six concentrations
X-Gal, também conhecida como 5-bromo-4-cloro-3-indoxil-β-D-galactopiranosídeo é um glicosídeo artificial análogo à lactose e um substrato cromógeno para a enzima β-galactosidase. A enzima β-galactosidase hidrolisa X-Gal, formando galactose e 5-bromo-4-cloro-3-indoxil.
the activation of senescence-associated β-gal, a specific biomarker of cell senescence, was detected by the accumulation of blue-violet X-gal crystals in FLO-treated L cells (Fig. 5D). The morphology.
strongly recommend that you place the X-gal in a sealed desiccated container.
use the solvent DMSO, but in our experience it is critical that the DMSO be.
X-GAL is soluble in N, N-dimethylformamide or dimethylsulfoxide (DMSO). Together with appropriate bacterial cloning vectors, host strains, and IPTG, X-GAL provides an easy way to distinguish between.
We compared the aging BMSCs treated with apocynin (100 μM) with the DMSO-treated negative control and.
Assessment of senescence-associated β-galactosidase (SA-β-gal) activity SA-β-gal was.
Golden Mutagenesis: An efficient multi-site-saturation mutagenesis approach by Golden Gate cloning with automated primer design – Final reaction mixtures consisted of 3% (v/v) DMSO; 1x concentrated Phusion Green HF buffer (ThermoFisherScientific.
A volume of 50 µl was used for plating on LB-agar plates (+X-Gal, +spectinomycin.